Light Microscopy

The University of Richmond maintains a wide variety of instruments to support research and classroom experimentation.

• The Olympus IX83 is an inverted compound microscopes for fast acquisition of live cell fluorescent microscopy. The motorized focus, filter wheel, and stage allow for z-series acquisition over time and over multiple locations. Four fluorochromes can be imaged including blue, green, red, and far red. The system is equipped with a Hamamatsu Orca Flash 4.0 camera for exceptional sensitive, low noise to signal, fast acquisition, and high resolution (4 megapixels).

• The Olympus BX61 is an upright compound microscope built for live cell imaging with fast changing motorized filters, Z-stage, and objective turret. The system is fully automated for single images, timelapse imaging, and z-series imaging with Micromanager software. The microscope is equipped with a high resolution Hamamatsu Orca-ER digital camera which images at 1.37 linear megapixels. Fluorescence images can be obtained in the blue, green, and red ranges.

• The Zeiss Imager Z1 is an upright compound fluorescent microscope. This system offers epifluorescent imaging of green, red, and blue fluorochromes with both mercury and LED lighting. A motorized multi-slide stage and Zeiss software allows for multi-point and montage imaging.

• The Olympus IX51 is an inverted compound microscope equipped with phase contrast and DIC for imaging live cells as well as a microinjector. Single or timelapse images are captured with an Infinity 2 color camera at up to 1616x1216 resolution.

• Nikon Diaphot inverted fluorescent microscope equipped with blue, green, and red filters. A Cool Snap HQ2 operated by micromanager provides imaging capability.

• The Olympus IX71 is an inverted fluorescent microscope with blue, green, and red filters. A QImaging CMOS camera operated by micromanager provides imaging capability.

• The Nikon E600 is an upright compound fluorescent microscope. The system is equipped with filters for blue, green, and red imaging on a ProgRes color camera. This system can obtain single images and timelapse series.

• The Zeiss NeoLumar dissecting microscope is equipped with motorized filter wheel for blue, green, and red fluorescent imaging and motorized focus for z-series imaging. Images are obtained with a Zeiss AxioCam MRc color camera.

• The Nikon Eclipse E400 is an upright compound microscope with 4x, 10x, 40x, and 60x objectives. This system is equipped with a Sanyo VCC 3972 high resolution video camera (460x400 TV lines). The images can be projected to show the entire class a sample.